Figure 6. Glucotoxic/hyperglycemic stress reduced Survivin protein levels in pancreatic β-cells. MIN6 cells were transfected with pCMV6 vector or pCMV6-myc-PAK1 plasmid. After 48 h incubation, cells were washed twice with and incubated for 2 h in freshly prepared modified Krebs-Ringer bicarbonate buffer. Cells were stimulated with 20 mM D-glucose for 20 min. Cells were then washed twice before harvesting, and whole cell lysates were subjected to immunoblotting with antibodies indicated. Data represent the average ± SE for 3 independent experiments; *p < 0.05 vs. basal.