Figure 7. (A) Decreased [³H]methyl-thymidine incorporation in islets from PAK1^+/− Het mice. [³H]Methyl-thymidine was added to pools of about 200 islets for 18 h. The islets were washed three times with cold medium. The amount of [³H]thymidine incorporated into DNA was measured by liquid scintillation counting and normalized to total cellular protein. Data represent the average ± SE for 3 independent experiments. *p < 0.05, vs. WT. (B) Overexpressing Survivin restored impaired-proliferation of MIN6 cells induced by loss of PAK1 in pancreatic MIN6 β-cells. MIN6 cells were transfected with PAK1 siRNA (siPAK1) or control (siCon) oligonucleotides with MIEG3 vector or Survivin plasmid as described in the Materials and Methods. Thirty-six hours after transfection, [³H]methyl-thymidine was added for 2 h, and the amount of [³H]thymidine incorporated into DNA was measured as described in the Materials and Methods. Data are the average ± SE from 3 independent experiments. *p < 0.05 vs. siCon; # p < 0.05 vs. siPAK1.