Figure 5. Basal neutral sphingomyelinase (NSMase) expression in WT, RIP-iPLA₂β-Tg and iPLA₂β-KO islets. Total RNA was isolated from islets from the three groups and cDNA generated for qRT-PCR analyses of NSMase message expression. (*^#Significantly different from corresponding WT group, p < 0.05 and p < 0.005, respectively; n = 3).