Fig. 5.

Effects of GSH or Trolox treatment on CEES-induced oxidative stress and oxidative DNA damage in JB6 cells and fibroblasts. (A and B) JB6 cells and fibroblasts were treated with 10 mM GSH or 800 µM Trolox for 1 h before 0.5 mM CEES exposure for 4 h and the cellular ROS were determined by incubation with DHE for 30 min followed by flow cytometry as described under Materials and methods. (C and D) To assess the effect of 1 h pretreatment with 10 mM GSH or 800 µM Trolox on the oxidative DNA damage caused by 0.5 mM CEES exposure, DNA was isolated after the desired treatments and 8-OHdG ELISA was conducted as described under Materials and methods. Data are presented as means ± SEM, n = 3. *P<0.001, $P<0.005 compared to 0.5 mM CEES exposure. UC, untreated control; DMSO, vehicle control.