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. 2013 Apr 14;111(6):1139–1153. doi: 10.1093/aob/mct080

Fig. 3.

Fig. 3.

Ta-PHR1-A1 homodimer confers transcriptional activation of Ta-PHT1.2. (A) BiCF demonstrated that Ta-PHR1-A1 formed a homodimer in vivo. Images were captured 20 h after transient expression under an Olympus confocal microscope. Scale bars = 20 µm. (B) Ta-PHR1-A1 activated the expression of the reporter gene GUS driven by the Ta-PHT1.2 promoter. Left panel: the growth of the yeast cells on SD-Leu/Trp media. Right panel: GUS staining. 1, pAD-GAL4-Ta-PHR1-A1 + pBD-Ta-PHR1- A1-proTa-PHT1.2::GUS; 2, pAD-GAL4-Ta-PHR1-A1 + pBD-GAL4-Ta-PHR1-A1; 3, pAD-GAL4 + pBD-proTa-PHT1.2::GUS. (C) GUS activities of the yeast cells from (B); ** indicates that the difference was significant at the 0·01 level.