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. Author manuscript; available in PMC: 2013 May 23.
Published in final edited form as: Mol Cancer Ther. 2009 Aug 11;8(8):2356–2365. doi: 10.1158/1535-7163.MCT-08-1183

Figure 2. Immuno-labeling of MC4R and CCK2R and the distribution of heterobivalent ligand labeling in MC4R and CCK2R expressing cells.

Figure 2

A & B. Images of Hek293 cells, that stably expressed both the CCK2R and MC4R, labeled with antibodies against the MC4R and CCK2R receptors. C & D. Images of cells incubated for 3 minutes with 0.8 nM of Cy5 labeled MSH-CCK ligand, then washed with ligand free media. The ligand distribution was determined immediately following the rinse (C), and 7 minutes thereafter (D). Scale bar = 20 μM for both images pairs.