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Glycine oxidase: reaction and enzymatic activity assay. (A) Reaction catalysed by glycine oxidase. The carbons of the molecules were numbered. (B) Glycine oxidase activity (upper bars), butanol (dark gray columns) and isobutanol (pale gray columns) for the wild type and modified yeast strains overexpressing the bacterial goxB gene optimized for the yeast codon usage. The data presented here are representative of three independent experiments.
