Table 1.
β-Isopropylmalate dehydrogenase activity measured with assay coupling the reactions catalyzed by Mls1 and Leu2
|
Gene |
β-IPMD Activity (U/mg prot) |
||
|---|---|---|---|
| MLS1 | DAL7 | LEU2 | |
| + |
+ |
++ |
0.098 ± 0.010 |
| + |
+ |
- |
0.023 ± 0.001 |
| + |
- |
++ |
0.019 ± 0.002 |
| + |
- |
- |
0.016 ± 0.002 |
| - |
+ |
++ |
0.021 ± 0.002 |
| - | + | - | 0.018 ± 0.002 |
The symbols “+” indicate the wild type copy or “++” the overexpression of the corresponding gene in the table. The symbol “-” indicate the deletion of the gene. The data presented here are representative of three independent experiments.