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. 2013 May 23;9(5):e1003516. doi: 10.1371/journal.pgen.1003516

Figure 4. Ikbkap is required for XY pairing and DSB repair.

Figure 4

(A) Representative images of immunostaining of meiotic chromosome spreads of control and CKO pachytene spermatocytes co-stained by anti-SYCP3 (green) and anti-γH2AX (red) antibodies. Areas surrounding sex chromosome are highlighted in dotted rectangles and are magnified in the right panels. Arrows indicate weak γH2AX signals on autosome. CKO spermatocytes exhibit XY synapsis defects, which were classified into three types. Type I: wide separation of X and Y chromosomes. Type II: illegitimate association of the X chromosome to autosomes. Type III: ectopic distribution of γH2AX signals on part of autosomes. Bar, 10 µm. (B) Distribution of the three types of XY synapsis defects in CKO testes (n = 302 nuclei, three animals for each genotype were analyzed). (C) Representative images of chromosome spreads from control and CKO spermatocytes co-stained with, RAD51 (green) and SYCP3 (red) antibodies or SYCP3 (green) and H1t (red) antibodies, or MLH1 (green) and SYCP3 (red) antibodies. Bar, 10 µm. Arrows indicate sex chromosome.