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. 2013 May 23;9(5):e1003383. doi: 10.1371/journal.ppat.1003383

Figure 1. Identification of HLA-C*0702-restricted IE-1-specific CD8+ T cells.

Figure 1

(A) Reactivity of T cell clone ALT#127 to titrated IE-1 peptides in IFN-γ ELISA (20 000 clonal T cells and 50 000 autologous peptide-loaded CD40-stimulated B cells per well in duplicates). (B, C) HLA restriction of the IE-1 CRV epitope. WI-38 fibroblasts were transfected with HLA-B*0702 or C*0702 and infected with MVAs encoding pp65 (B) or IE-1 (C), alongside with negative controls (no infection and wild-type (wt) MVA infection). Cells were then coincubated overnight with T cell clone F37#5 specific for pp65/TPR/B*0702 (B) or IE-1/CRV-specific T cell clones (C) from 3 different donors (AJJ, AJU and ALT). For each condition, 10 000 fibroblasts and 10 000 T cells were coincubated in 3 replicates. Supernatants were analyzed in an IFN-γ ELISA. (D) The reactivity of T cell clone AJU#90 to IE-1-expressing or control mini-LCLs from donors with various HLA types was tested in IFN-γ ELISA (10 000 T cells and 20 000 mLCLs in two replicates). For full HLA class I types, see Table S1.