Figure 7. Presentation of IE-1 to CD8+ T cells after infection with endotheliotropic HCMV.

MRC-5 fibroblasts were infected with HCMV TB40-BAC4, not infected (n.i.) or peptide-loaded (n.i.+peptide), and their recognition by T cell clones specific for the pp65 epitope NLV/A*0201 and the IE-1 epitopes CRV/C*0702 and VLE/A*0201 was analyzed. Fibroblasts were cultured in standard medium alone (A), with IFN-γ from 1 hour after infection (B), or were pretreated with IFN-γ 72 hours before infection (C). Infection was performed at moi = 5, and effector assays were set up 48 hours post infection (10 000 fibroblasts and 20 000 T cells per well). IFN-γ secretion was measured by ELISA. Data are shown as mean+SD of triplicate samples.