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. 2013 May 23;8(5):e63281. doi: 10.1371/journal.pone.0063281

Figure 2. Silencing ACS genes in tomato does not compromise Mi-1-mediated resistance to root-knot nematodes.

Figure 2

Two-week-old tomato plants cvs. Moneymaker (mi/mi) and Motelle (Mi-1/Mi-1) were used in agroinfiltration of tobacco rattle virus (TRV) empty vector, and cv. Motelle was used with TRV containing a portion of Mi-1 (TRV-Mi-1) or containing 1-aminocyclopropane-1-carboxylic acid synthase (ACS) constructs (TRV-ACSI and TRV-ACSII), which were either individually or simultaneously agroinfiltrated (TRV-ACSI+II). Three weeks after agroinfiltration, plants were infected with 10,000 second-stage juvenile root-knot-nematodes and evaluated for nematodes reproduction 8 weeks later. Dots represent the number of egg masses counted on a single root system (n = 18–25). Two independent experiments were performed with similar results and data from one are presented.