Figure 5. Enrichment of TF motifs in Runx1 bound genomic regions.

(A) RUNX motif binding energy is correlated with RUNX1 ChIP-seq occupancy. The indicated RUNX motif was inferred directly from Runx1 occupancy peaks (Methods). Box plots depict the distributions of motif binding energies (Y-axis) in groups of regions with increasing Runx1 ChIP-seq coverage (binding level) (X-axis). The regions that passed the threshold and were thus defined as Runx1 bound are shown in dark blue boxes. The outliers represent values more than 90^th percentile. (B) ETS binding motif is correlated with Runx1 ChIP-seq occupancy. An ETS motif was inferred directly from its association with Runx1 bound regions and analysis was conducted as in (A). The correspondence between Runx1 occupancy, GATA and AP-1 motifs binding energies was analyzed as in (A) and is presented in (C) and (D). Box plots depict the distributions of motif binding energies (Y-axis) in groups of regions with increasing Runx1 ChIP-seq readout coverage (X-axis).