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. 2013 May 23;8(5):e64248. doi: 10.1371/journal.pone.0064248

Figure 6. Runx1 down-regulates AP-1 expression in maturating MK.

Figure 6

(A) Runx1 binding pattern surrounding genome loci of AP-1 TF. UCSC browser normalized tracks of Runx1 (blue) and non-immune serum (black) ChIP-seq traces surrounding Fos, Fosb, Jun and Junb loci. Red asterisks mark genomic region that were bound by Runx1 in early stages of MK-cell line differentiation[8]. (B). RT-qPCR analysis of Fos, Fosb, Jun and Junb expression in MKRunx1L/L (MKL /L) or MKRunx1−/− (MK−/−). Data represent the mean ± SD of three independent experiments performed in triplicates. The increased expression of Fos, Fosb Jun and Junb in MKRunx1−/− (MK−/−), relative to MKRunx1L/L (MKL /L), was significant [P = 0.04, 0.004, 0.001 and 0.01, respectively]. PCR primers used are listed in Table S2.