Skip to main content
. 2013 May 23;9(5):e1003366. doi: 10.1371/journal.ppat.1003366

Figure 1. Infected CD14 (+) cells express latency associated transcripts and stable DNA copy number after 14 days in culture.

Figure 1

(A) RT-qPCR analysis for the expression of IE1/IE2, LUNA and UL138 mRNA in infected CD14 (+) monocytes. CD14 (+) cells were infected with FIX BAC virus and total cellular RNA was harvested various days post infection and subjected to RT-qPCR using TaqMan primers and probes specific for IE1 and IE2 mRNA. Inset figure: Detection of viral genomic DNA in infected CD14 (+) monocytes by PCR. Lanes: 1, uninfected; 2, 5-day post infection; 3, 18 day post infection. Primers used were specific for the TR region of the genome. (B) CD14 (+) monocytes infected cell viral genome copy number. Cells were harvested at various days post infection and subjected to qPCR using primers and probes specific for the HCMV viral chromosome. Absolute viral genome copy number was determined by comparing to a standard curve and calculated on a per cell basis. Error bars are the standard deviation of the mean for 4 separate wells.