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. 2013 May 23;9(5):e1003366. doi: 10.1371/journal.ppat.1003366

Figure 2. RNA-Seq analysis of HCMV infected CD14+ cells.

Figure 2

(A) HCMV transcriptome 5 days post infection. (B) HCMV transcriptome 18 days post infection. Transcript analysis was performed using CLC Bio Genomics Workbench software. Peaks were calculated and determined based on 4 independent experiments and using a minimum of 20 read cut off and pValue of <0.05 was used to determine peaks shown. Peaks identified from 18 day CD14 (+) latent infection were: UL138, UL95, UL87, UL84, UL52, UL50, UL44, LUNA, RNA4.9 and RNA2.7. Peak heights are indicative of relative transcript accumulation in infected cells. Arrows indicate direction of transcripts. Inset: RT-PCR evaluation of identified ORFs during lytic or latent infection. Negative controls for latent infection: IE2, UL13 and US21. CycloA = cyclophillin A. (C) HCMV transcriptome upon reactivation of latently infected CD14 (+) monocytes with treatment of IL-6 and culturing cells on a surface that allows for cell attachment.