Figure 5. UV inactivation of virus abrogates accumulation of IE2 and latency associated transcripts in CD14 (+) monocytes.

CD14 (+) monocytes were infected with either wt FIX BAC virus or UV inactivated virus. Total cellular RNA was harvested at 1 hr, 5 and 10 days post infection and qPCR was performed to detect transcripts IE2, UL44, UL84, LUNA, UL138, lncRNA 2.7 and lncRNA4.9. Transcript fold increase was determined using mock infected as reference. 3 separate experiments were preformed and error bars are the SD of the mean.