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. 2013 May 23;9(5):e1003366. doi: 10.1371/journal.ppat.1003366

Figure 10. A plasmid clone containing the circularized TR region of HCMV genome is maintained in latently infected CD14 (+) monocytes.

Figure 10

(A) Schematic showing the circularized region of the HCMV genome subcloned into the plasmid vector pGEM7zf(−). (B) Schematic showing the development of a latent replication/maintenance assay in CD14 (+) monocytes. (C) Infection/transfection protocol used to evaluate plasmid maintenance in latently infected CD14 (+) monocytes. (D) Southern blot of a Gardella gel containing samples from latently infected CD14 (+) monocytes transfected with plasmids pGEM7zf(−), pOriLyt, pTR or pTR from uninfected CD14 (+) cells 26 days post transfection. Arrows indicate the presence of two bands in the pTR lane at 15 days post transfection, 25 days post infection.