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. 2013 May 23;8(5):e64362. doi: 10.1371/journal.pone.0064362

Figure 1. Experimental design for evaluation of Se, green tea and the combination diet for chemopreventive effects against colonic carcinogenesis in an AOM-induced rat CRC model.

Figure 1

ACF study (A): groups of rats (n = 15) were fed control, Se, green tea or diet containing Se and green at age of 5 wk. 2 wk later, rats were given 15 (mg/Kg/body weight) AOM, once a week for 2 wk. 8 wk after last AOM treatment, rats were euthanized and colons were evaluated for ACF; normal-appearing crypts were also examined for β-catenin, COX-2 and Ki-67 expression. Tumor study (B) : groups of rats (n = 25) were fed control, Se, green tea or diet containing Se and green at age of 5 wk. Rats were given two weekly AOM treatments similar to ACF study. 30 wk after AOM treatment, rats were euthanized and colons were historically evaluated for tumor outcomes; normal-appearing crypts were also examined for β-catenin, DNMT1, Ac-H3 as well as SFRP5 and Cyclin D1 expression.