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. 2013 May 23;8(5):e64160. doi: 10.1371/journal.pone.0064160

Figure 4. In vivo survival and differentiation of hiPS-NPs in the ischemic brain.

Figure 4

(A–D) hiPS cells survive and differentiate to neurons 28 days after transplantation in the cortex penumbra region of stroke animals. The core region is delineated in (A) with the Hoechst 33342-positive hiPS cell-derived neurons residing in the penumbra. hiPS cell-derived neurons are identified by co-labeling Hoechst-33342 and NeuN. (D) is a magnified image of the marked area in (C). Blue is Hoechst-33342, green is Collagen IV (vessel marker) and red is NeuN. (E, F) are further magnifications to show the co-labeled Hoechst 33342-positive NeuN-positive hiPS cell-derived neurons. A Hoechst 33342-negative NeuN-positive host neuron is also shown in (F). Bar = 50 µm for A to C, 20 µm for D, 30 µm for E and 10 µm for F. (G–H) Nissl staining of brain sections representing brains of hiPS-NP transplanted animals 6 and 12 months after transplantation showing no indication of tumor formation. Asterisks indicate stroke location. N = 6 per group at each time point. (I) The in vivo experimental design for hiPS-NP transplantation and post-stroke experiments.