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. 2013 May 23;8(5):e65045. doi: 10.1371/journal.pone.0065045

Figure 5. Effect of phosphomimicking negative charges on di-domain self-association.

Figure 5

(A) Representative SDS-PAGE strips from disulfide trapping experiments of di-domain mutated at sites known to be phosphorylated by GSK3 and SRPK1. The arrow denotes the position of disulfide-linked di-domain on the gel. Strips from other mutants are included in the supplemental material. (B) Quantification of the self-association potential of all phosphomimicking mutants from this study. Bars represent average results from three independent experiments.