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. 2013 May 23;8(5):e63672. doi: 10.1371/journal.pone.0063672

Figure 4. Cytokine profiles in the ex vivo and in vitro samples treated with vitamin D3.

Figure 4

Sequential data of quantification of 3 cytokines (IFN-γ, IP-10 and RANTES) during 1(OH) vitamin D3 pre-treatment (pre vs 0w), 1(OH) vitamin D3/Peg-IFN/RBV therapy are shown (A). Dotted lines indicate the data of each subject. Black lines indicate the averaged data. Error bars indicate standard deviation. The data from IL28B (T/T) subjects or IL28B (T/G or G/G) subjects are shown in the independent graphs (A). Comparisons of the amounts of 3 cytokines (IFN-γ, IP-10 and RANTES) between the 1(OH) vitamin D3/PEG-IFN/RBV group (VitD3+standard of care (SOC)) and Peg-IFN/RBV group (SOC) at 0 weeks and 12 weeks after the start of Peg-IFN/RBV treatment are shown (B). Analysis of the changes in the amounts of the 3 cytokines (IFNγ, IP-10 and RANTES) during 12 weeks treatment of Peg-IFN/RBV is shown. Schema of in vitro-analysis of co-culture is shown (B). alfa-calcidol: 1(OH)vitamin D3 and calcitriol: 1,25(OH)vitamin D3 were used to analyze the cytokine production in vitro. Black bars indicate the data from samples treated with alfa-calcidol. Gray bars indicate the data from samples treated with calcitriol. *p<0.05.