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. 2013 May 23;8(5):e63672. doi: 10.1371/journal.pone.0063672

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© 2013 Kondo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Representative dot plots of CD3⁺CD4⁺CD25⁺IL7R⁻ (Tregs) and CD3⁺CD4⁺CXCR3⁺CCR5⁺ (Th1 cells) are shown. (A) Frequencies of Th1 and Tregs among the 4 groups (IL28B T/T vitamin D3/Peg-IFN/RBV, IL28B T/G or G/G vitamin D3/Peg-IFN/RBV, IL28B T/T Peg-IFN/RBV, and IL28B T/G or G/G Peg-IFN/RBV) are shown. (B) Comparison of the T-bet and IFN-γ mRNA expression between subjects treated with vitamin D3/Peg-IFN/RBV therapy and those treated with Peg-IFN/RBV therapy. Each group included 5 patients. Total mRNA was extracted from isolated CD4⁺ T cells. The relative expression levels are shown in bar graphs. The statistical analysis was carried out by independent student t-test.