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. 2013 May 23;8(5):e64770. doi: 10.1371/journal.pone.0064770

Figure 4. miR172b regulates post-germinative growth arrest by directly targeting SNZ.

Figure 4

Wild-type seeds were germinated on MS with or without treatments, and materials were collected at 2 to 4 days after stratification. (A) SNZ expression in response to ABA (5 µM); (B) SNZ expression in response to NaCl (100 mM); (C) SNZ expression in response to mannitol (300 mM); (D) and (E) snz-1 seedlings showed delayed greening under ABA treatments; Photographs were taken 7 days after stratification. Bar = 4 mm. All the experiments were performed three times with three replicates. Error bars denote±SD. Student's t test was performed, and the statistically significant treatments are marked with asterisks. (*) P<0.05, (**) P<0.01 and (***) P<0.001.The expression level was normalized against the reference gene GAPC. All the experiments were performed three times with three replicates. Error bars denote±SD.