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. 2013 May 23;8(5):e63594. doi: 10.1371/journal.pone.0063594

Figure 6. Pre-steady-state kinetics of 3α-HSD/CRs.

Figure 6

The stopped-flow progress curves for 3α-HSD/CRs catalysis of the reaction of the undeuterated (blue diamond) and deuterated (black circle) androsterone with NAD+. Reactions were performed in 1 µM WT, 1 mM NAD+, 17.5 µM undeuterated or deuterated androsterone at 0.1 M Caps, pH 10.5. The line represents the fit of the data points to Equation 10, giving an apparent value for kobs of 309 s−1 for the reaction catalyzed by 3α-HSD/CRs with the deuterated androsterone.