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. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: J Mol Biol. 2011 Jul 22;410(4):698–715. doi: 10.1016/j.jmb.2011.04.025

Figure 2.

Figure 2

NCp7 association with the anticodon stem and loop domain of htRNALys3UUU. The binding of hASLLys3UUU to NCp7 was monitored by observing the change in fluorescence (quenching) of the protein’s one tryptophan (W37). (a) NCp7 (75 nM) titrated with increasing concentrations (0 – 3 μM of the hASLLys3UUU-mcm5s2U34;ms2t6A37 (●) and the unmodified hASLLys3UUU (■). (b) NCp7 binding of the HIV-1, serotype-G, viral Stem and Loop 1. The quenching of the NCp7 W37 was monitored in a single experiment when titrated with a truncated construct of the A-rich HIV-1, serotype-G, viral Stem and Loop 1 (VSL-1 with VGL).