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. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: J Mol Biol. 2011 Jul 22;410(4):698–715. doi: 10.1016/j.jmb.2011.04.025

Figure 4.

Figure 4

NCp7 disruption of the htRNALys3UUU anticodon stem and loop domain compared to thermal denaturation. An unmodified anticodon stem and loop domain construct (hASLLys3UUU) with a 5′-conjugated fluorescein and a 3′-conjugated dabsyl was titrated with NCp7 and the increase in fluorescence monitored (●). The hASLLys3UUU was half “melted” at an NCp7 to RNA ratio of ~2. In addition, an unmodified hASLLys3UUU (without fluorescein or dabsyl) was thermally denatured/renatured from 20 to 95 °C and the UV absorbance at 260 nm was recorded, averaged and normalized (percent absorbance, ●). The temperature at which the sample was half-melted was ~40 °C.