Figure 3.

The hmtASL^Met_CAU NOSEY spectra. NOESY spectra of hmtASL^Met_CAU-Ψ₂₇;f⁵C₃₄ (a) and hmtASL^Met_CAU-Ψ₂₇ (b). NOSEY spectra of the hmtASL^Met_CAU in D₂O were recorded at 25° C with a mixing time of 400 ms. The H1′ and H5 to aromatic portion of the spectra are shown. The sequential H1′-aromatic connectivities are indicated, as well as the unusual NOEs connectivities between A₃₅H2 – A₃₇H1′ (blue) and A₃₅H8 – C₃₃H1′ (red).