Figure 3. Identification of GATA3^hi ILCs in the bone marrow.

(A,B) Expression of Id2 (GFP) (grey) by the indicated cell subsets obtained from spleen or intestinal lamina propria of Id2^Gfp/+ mice. Open histograms represent analysis of the same population from control mice (A). (B) Mean fluorescence intensity (MFI ± SEM; n=4) of Id2 (GFP) expression in comparison to control mice by the indicated cell subsets isolated from the intestinal lamina propria.

(C) Id2 (GFP) and GATA3 (GFP) expression (grey) by the indicated bone marrow cell populations (HSC/MPP: Lin⁻CD127⁻Sca1⁺Kit⁺; CLP: Lin⁻CD127⁺CD135⁺Sca1^loKit^lo). Open histograms represent analysis of the same population from control mice. Numbers indicate percentage of positive cells.

(D) Analyses of Sca1, Id2 (GFP) and GATA3 expression by Lin⁻ cells. The dot plots to the right show CD127 and CD25 expression by electronically gated Sca1^hiId2^hi (top) or Sca1^hiGATA3^hi cells (bottom). Numbers represent percentage of cells in the indicated gates.

(E) Flow cytometry analysis of CD44 and CD90 expression (grey) by bone marrow LSI cells. Open histograms show staining with an isotype-matched control antibody.

(F) The indicated mouse strains were analyzed for the presence of bone marrow Lin⁻ Sca1^hiGATA3^hi cells. Numbers represent percentage of cells in the indicated gates.

Data are representative of at least three independent experiments. ** p ≤ 0.01, *** p ≤ 0.001. LSI: Lin⁻Sca1^hiId2^hi bone marrow cells. See also Figure S2.