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. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: Immunity. 2012 Oct 11;37(4):634–648. doi: 10.1016/j.immuni.2012.06.020

Figure 7. GATA3 controls cell fate and maintenance of ILC2.

Figure 7

(A) Analyses of RORγt and KLRG1 expression by YFP+ (Cre-on) and YFP− (Cre-off) fractions of CD45+CD3−CD19− lamina propria cells from the indicated mouse strains. Numbers represent percent cells in quadrant.

(B) GATA3 expression by intestinal YFP+ (Cre-on) RORγt+ ILCs of Gata3+/+ (grey) or Gata3f/f (open) animals.

(C) Expression of GATA3 and KLRG1 by YFP+ (Cre-on) and YFP− (Cre-off) fractions of CD45+CD3−CD19− intestinal lamina propria cells from the indicated mouse strains. Numbers represent percent cells in gate.

(D,E) Percentage (± SD; n=4) of KLRG1+YFP+ cells (D) and of YFP+ cells (E) among CD45+CD3−CD19− lamina propria lymphocytes from the indicated mice.

(F) Lamina propria lymphocytes from the indicated mouse strains were stimulated for 4 h with PMA and ionomycin. IL-5 and IL-13 production was analyzed by intracellular cytokine staining and flow cytometry analysis of electronically gated CD45+CD3−CD19− CD90+ lamina propria lymphocytes. Numbers indicate percent cells in quadrant.

(G) Highly purified YFP− ILC2 were cultured on mitotically inactivated OP9 feeder cells in the presence of IL-2, IL-7 and 4-OH tamoxifen. After 7 days, cells were analyzed for expression of YFP and KLRG1. Numbers represent percentage of cells in the indicated gates.

(H) Expression of Sca1 and YFP among Lin− cells of the bone marrow from the indicated mouse strains.

(I) Expression of CD25 and Sca1 by Lin− bone marrow cells. Numbers indicate percentage of cells in quadrant. The lower panel shows the expression of YFP (Cre-on) in electronically gated Lin−CD25+Sca1hi bone marrow cells of the indicated mouse strains. Numbers indicate percentage of YFP-positive cells.

(J) Highly purified YFP+ ILC2P were cultured on mitotically inactivated OP9 feeder cells in the presence of IL-2, IL-7 and 4-OH tamoxifen. After 7 days, the cells were analyzed for expression of YFP and KLRG1. Numbers represent percentage of cells in quadrants.

All data are representative of at least two independent experiments. *** p ≤ 0.001, n.s.: not statistically significant. See also Figure S5.