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. 2013 May 24;4:125. doi: 10.3389/fmicb.2013.00125

Figure 1.

Figure 1

Plasmid profile and Southern hybridization of oqxB and qnrS1 in HUE1, its oqxAB- and/or qnrS1-cured strains, and oqxAB− and/or qnrS1−re-introduced strains. (A) Plasmid profile. Electrophoresis was performed at 100 V for 70 min with a 0.8% agarose gel. (B) Southern hybridization with the oqxB probe. (C) Southern hybridization with the qnrS probe. Lane 1, HUE1; lane 2, HUE1A2; lane 3, HUE1A; lane 4, HUE1A-Curoqxqnr; lane 5, HUE1A-Reoqx; lane 6, HUE1A-Reqnr; lane 7, HUE1A-Reoqxqnr; lane 8, HUE1; lane 9, HUE1B-Curoqx; lane 10, HUE1B-Curoqxqnr; lane 11, HUE1B-Reqnr; lane 12, HUE1B-Reoqx; lane 13, HUE1B-Reoqxqnr; R, DNA Molecular Weight MarkII, DIG-labeled: r, BAC-Tracker Supercoiled DNA Ladder.