Table 1.
Sequences of primers used for PCR and DNA sequencing in this study.
| Gene | Forward primer or fluorescent probe (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| acrA | acrA-1F (caccggcagtttgaggatcg) | acrA-1R (gcgcggatcaatctggctta) |
| acrA-2F (gtcgttgctggactgggtca) | acrA-2R (atgaacaaaaacagagggtttacg) | |
| acrB | acrB-1F (tcaatgatgatcgacagtatggct) | acrB-1R (ggaacaactggcgagcaaac) |
| acrB-2F (agcggaacgaccagcataac) | acrB-2R (gcgggacgtggtcagaatac) | |
| acrB-3F (ccagcctggtcaatcagctc) | acrB-3R (gcgtgttatggcggaagaag) | |
| acrB-4F (cgaataccgccgacagtacc) | acrB-4R (ggatgaacccgaatgagctg) | |
| acrB-5F (caggattttgccgaactcttca) | acrB-5R (ataaccagcaagccgcaagc) | |
| acrE | acrE-F(atagccgaagttcgcccaca) | acrE-R (ctgcgggggtatcggtagtg) |
| acrF | acrF-1F (cagtcaggcgattggcgata) | acrF-1R (accaccgagccgtcactgtt) |
| acrF-2F (cagcgttaccagggcaacaa) | acrF-2R (attttgccgacgctgttggt) | |
| acrF-3F (cgctgcttaaacccgtctctg) | acrF-3R (cagtcgcggagagccataca) | |
| acrF-4F (cgctgggtgggacttacgtt) | acrF-4R (ttatcctttaaagcaacggcgga) | |
| acrR | acrR-F (atcagaacgaccgccagagg) | acrR-R (ttattcgttagtggcaggattacga) |
| acrS | acrS-F (ttacatgacacttaattcattcgtttga) | acrS-R (tgcacatcgctgccttcagt) |
| oqxAB | oqxAB-1F (acatttaccggaataaaaat) | oqxAB-1R (ggcgaggttttgatagtgga) |
| oqxAB-2F (acggtgtacgtctactttga) | oqxAB-2R (gtctcggcaatcactttcg) | |
| oqxAB-3F (gcgcgcggagtatcccggcg) | oqxAB-3R (ccgcatccttattgttgagc) | |
| oqxAB-4F (atcgagatgggttccggtag) | oqxAB-4R (taaacggacggaaaatccag) | |
| oqxAB-5F (tggcggccctgctgttaaag) | oqxAB-5R (gataggtctgcagcgtaccg) | |
| oqxAB-6F (ctggacgtgcaggtcgatcg) | oqxAB-6R (gataaaggcgatggaggtcat) | |
| oqxAB-7F (gagctgtcgaagcagatcct) | oqxAB-7R (tgcgacccggtgccggaaat) | |
| qnrS | qnrSseq-F (ttagtcaggataaacaacaataccca) | qnrSseq-R (atggaaacctacaatcatacatatcgg) |
| rob | robA-F (catctggacgcccctgcatt) | robA-R (agccaatggccccagcatta) |
| soxSR | soxSR-F (gcgctattgccagggatggt) | soxSR-R (tgtgttgacgtcgggggaaa) |
| tolC | tolC-1F (cgggcaggttgtctggctta) | tolC-1R (ctggctcaagcgtgcctgta) |
| tolC-2F (gctgcgctgaatgtcgaaaa) | tolC-2R (tgcgtggcgtatggattttg) |