Table 3.

Fluoroquinolones and NAL susceptibilities of HUE1, transformants derived from DH5α, and HUE1mutants derived from plasmid curing and reintroduction.

Strain	PCR		MIC (mg/L)
	oqxAB	qnrS1	CIP	LVX	URX	STX	NOR	NAL
HUE1	+	+	4 (×4)a	8 (×16)	4 (×4)	2 (×16)	16	128
TRANSFORMATION OF pHFQ1 AND pHFQ2
DH5α	−	−	0.015	0.03	0.015	0.004	0.03	32
DH5α/pHFQ1	+	−	0.03	0.03	0.03	0.015	0.06	64
DH5α/pHFQ1(ΔoqxAB::hygr)	−	−	0.015	0.03	0.015	0.004	0.03	32
DH5α/pHFQ2	−	+	0.125	0.5	0.125	0.125	0.25	128
DH5α/pHFQ2(ΔqnrS1::hygr)	−	−	0.015	0.03	0.015	0.004	0.03	32
DH5α/pHFQ1-pHFQ2	+	+	0.25	0.5	0.125	0.25	0.25	>128
MUTANTS OBTAINED FROM PLASMID CURING
<Group A>
HUE1A and HUE1A2	+	+	2 (×2)	2 (×4)	2 (×2)	1 (×8)	8	64
HUE1A-Curoqxqnr	−	−	0.06 (×2)	0.125 (×4)	0.03 (×1)	0.03 (×4)	0.5	16
HUE1A-Reoqx	+	−	0.125 (×4)	0.125 (×4)	0.125 (×2)	0.06 (×8)	0.5	32
HUE1A-Reqnr	−	+	1 (×2)	1 (×4)	1 (×1)	0.5 (×4)	8	64
HUE1A-Reoqxqnr	+	+	2 (×2)	2 (×4)	2 (×2)	1 (×8)	8	64
<Group B>
HUE1B-Curoqx	−	+	0.25 (×1)	0.125 (×1)	0.125 (×1)	0.03 (×1)	0.25	4
HUE1B-Curoqxqnr	−	−	0.008 (×1)	0.008 (×1)	0.008 (×1)	0.004 (×1)	0.03	1
HUE1B-Reoqx	+	−	0.125 (×8)	0.06 (×8)	0.03 (×2)	0.03 (×8)	0.25	16
HUE1B-Reqnr	−	+	0.125 (×1)	0.125 (×1)	0.125 (×1)	0.03 (×1)	0.25	4
HUE1B-Reoqxqnr	+	+	1 (×4)	1 (×4)	0.5 (×2)	0.25 (×8)	4	32

^aReduction of MIC by PAβN (-fold),

^rresistance.