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. 2013 May 24;3:131. doi: 10.3389/fonc.2013.00131

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Copyright © 2013 Huang and Murphy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Characterization of IGF2 intron 3 CTCF binding motif methylation in spermatozoa and human fetal tissues. (A) Measurement of % methylation (y axis) for both CpG dinucleotides (CpG Site 1, CpG within the CTCF binding motif; CpG Site 2, CpG adjacent to the CTCF binding motif) in 16 individual human spermatozoa specimens (1–16 on x axis) and in human fetal liver (FL). The results from each of two assays are shown. (B) Box and whisker plot showing average percent methylation for the two CpG dinucleotides in the IGF2 intron 3 region across a panel of human conceptal tissues (boxes, 10th to 90th percentile). The number of individuals analyzed for each tissue type is indicated.