Fig. 3.

RT-PCR analysis for mRNA expression of the inflammation-related adhesion molecules in C, HaCaT cells (A, B) and HUVECs (C, D). The HaCaT cells and HUVECs were incubated for 6, 12, 24 and 48 hours after γSEA protein (100 ng/ml) treatment. (A, C) RT-PCR analysis for mRNA expression of E-selectin, ICAM-1, VCAM-1 and GAPDH was performed. (B, D) The density of each band was measured by a scanning densitometry and then expressed as the mean±standard deviation. The red box denotes significant upregulation of mRNA expression. M: marker, C: control, RT-PCR: reverse transcriptase-polymerase chain reaction, HUVECs: Human Umbilical Vein Endothelial Cells, γSEA: recombinant staphylococcal enterotoxin A.