. 2013 May 28;19(20):3007–3017. doi: 10.3748/wjg.v19.i20.3007

Table 2.

Concentrations of total glutathione, thioredoxin, nitrosothiols, and activity of glutathione peroxidase, malondialdehyde-thiobarbituric acid reactive substances, nitrosothiols, nitrotyrosine, and keratin-18 in the liver of rats

		Day 7	Day 14	Day 30
Glutathione	S	34.3 ± 3.1	33.2 ± 1.4	25.3 ± 1.2^a
34.0 ± 1.3 nmol/mg prt	R	35.1 ± 1.2	33.7 ± 1.0	30.9 ± 1.1^a ^c
Thioredoxin	S	14.2 ± 1.0^a	16.3 ± 2.4^a	5.2 ± 0.7
5.6 ± 0.9 nmol/mg prt	R	11.3 ± 1.0^a	14.4 ± 1.0^a	6.2 ± 0.7^c
Nitrosothiols	S	32.9 ± 3.5^a	30.0 ± 2.2^a	13.0 ± 1.2^a
16.8 ± 4.7 pmol/mg prt	R	35.4 ± 2.7^a	27.3 ± 2.2^a ^c	15.8 ± 1.5^a ^c
MDA-TBA	S	17 ± 4^a	39 ± 8^a	92 ± 10^a
12 ± 3 nmol/L	R	12 ± 3	19 ± 4^a ^c	41 ± 9^a ^c
Nitrosothiols	S	73 ± 15	79 ± 12^a	88 ± 10^a
62 ± 10 nmol/L	R	67 ± 10	69 ± 10	74 ± 11^a ^c
Nitrotyrosine	S	6 ± 2	10 ± 3^a	14 ± 3^a
6 ± 1 nmol/L	R	5 ± 1	5 ± 2^c	10 ± 2^a ^c
K-18	S	222 ± 21	233 ± 16^a	289 ± 20^a
198 ± 20 U/L	R	210 ± 10	213 ± 13^c	240 ± 17^a ^c
GPx activity	S	9.3 ± 1.2^a	21.1 ± 3.7^a	13.7 ± 1.8^a
4.5 ± 0.4 nmol NADH/min/mg prt	R	6.3 ± 0.9^a,b	20.8 ± 1.4^a	16.6 ± 2.6^a,b

The liver of rats fed a choline-deficient (CD) diet without (S)/with (R) Realsil or a normal choline-supplemented diet (controls). Values from controls are reported in the first column under the parameter. Data are mean ± SD of n = 5 rats per group at each time point.

P < 0.05 vs control rats;

P < 0.05 vs rats on CD diet without Realsil at the same time point. GPx: Glutathione peroxidase; MDA-TBA: Malondialdehyde-thiobarbituric acid reactive substances; K-18: Keratin-18; prt: Protein.