Fig. 7.

Heat maps of derived peak ratios retain polarization sensitivity when rotated, even with reduction of objective numerical aperture (NA). Bright field images reference the scanned areas at 20x ($\mathrm{NA}=0.4$) and 50x ($\mathrm{NA}=0.75$). Color maps are set to universal scale within each quantity. (a) $\nu 1$ Phosphate/Amide I maps at 20x show mild intensity changes under rotation at the border between osteonal and interstitial tissue. (b) The effect on $\nu 1$ Phosphate/Amide I increases with increased NA in the 50x maps. (c) $\nu 1$ Phosphate/Proline shows no notable intensity changes with rotation at 20x. (d) $\nu 1$ Phosphate/Proline shows no notable intensity changes with rotation at 50x, but noise is increased.