TABLE 4.
Quantitative evaluation of the different steps of the microarray procedure for viral detection in brain samples from experimentally infected animals
| Sample | RNA (PFU/mL)a | cDNA (PFU/mL)a | WTA (PFU/mL)a | Call rateb (%) | BLASTN (Homologous Strain) | Divergencec versus homologous strain (%) |
|---|---|---|---|---|---|---|
| CCHFV IbAr10200 (mouse brain) | 1.9 × 104 | 2.0 × 105 | 1.9 × 109 | 82.6 | IbAr10200 | 1.9 |
| Nipah virus UMMC1 (monkey brain) | 3.08 × 105 | 4.4 × 105 | 2.3 × 109 | 60.9 | UM-MC1 | ND |
CCHFV, Crimean–Congo haemorrhagic fever virus; ND, not done.
a
Specific viral genetic material evaluated by quantitative PCR, expressed in equivalent PFU/mL.
b
Call rate for the detection of the strain/isolate on the microarray.
c
Percentage of divergence (531 bp region in the polymerase gene) against the sequence tiled on the microarray.