Figure 15.

Measured results of the biosensor of Kang et al. for the detection of the enzymatic reaction between horseradish peroxidase and a film of polyaniline (PAn) on a gold electrode in the presence of H₂O₂: i) CV curve (a) and reflectance change (ΔR) (b) as a function of a potential at a fixed incident angle of 62.2°; ii) SPR measurements of reflectivity versus incidence angle at varying electrochemical conditions 1) bare gold electrode 2) reduced PAn film after maintaining a potential of 0 V for 5 minutes 3) oxidized PAn film after maintaining a potential of 0.9 V for 5 min iii) SPR kinetic curves at a fixed angle of 62.8° resulting from the HRP/PAn reaction at various concentrations of H₂O₂; iv) the relationship between the slope of the ΔR-t curves from iii) and the H₂O₂ concentration [116].