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. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: Biochem J. 2012 Jul 15;445(2):229–236. doi: 10.1042/BJ20120053

Figure 3. ERK regulates SP-1-driven MMP-9 gene expression at S586.

Figure 3

(A) Whole cell lysates were obtained from WT and Rac1 null macrophages. (B) Macrophages were cotransfected with MMP-9-luciferase and either empty or constitutively active MEK1 in combination with either SP-1WT or SP-1S586A. n=3, *p < 0.05. (C) Macrophages were transfected with MMP-9-luciferase and twenty four hours later, cells were incubated with U0126 (10µM) or 0.1% DMSO for 6 hrs. Results show Mean±SEM of firefly luciferase normalized to Renilla. n=8, *p<0.05. (D) Rac1 null macrophages were co-transfected with MMP-9-luciferase and dominant negative (DN)-ERK and SP-1WT or SP-1S586A vectors. Results show Mean±SEM of firefly luciferase normalized to Renilla. n=3, *p < 0.05.