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. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: Biochem J. 2012 Jul 15;445(2):229–236. doi: 10.1042/BJ20120053

Figure 5. Decreased MMP-9 and SP-1 expression and ERK activation in asbestosis patients.

Figure 5

(A) WT and Rac1 null mice were intratracheally administered 100 µg chrysotile asbestos. Animals were euthanized at one or 28 days after exposure, and BAL was obtained. Active MMP-9 was determined by measuring the difference between total and pro-MMP-9 which were determined. N=4 (WT and Rac1 null); *p < 0.041. (B) Total RNA was isolated from alveolar macrophages obtained from normal subjects (n=3) or patients with asbestosis (n=3). MMP-9 mRNA expression was measured by real-time PCR. * p < 0.001. (C–D) Alveolar macrophages from asbestosis patients (n=3) and normal subjects (n=3) were lysed and subjected to immunoblot analysis for the determination of SP-1 and phosphorylated ERK.