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. 1973 Mar;51(3):577–583. doi: 10.1104/pp.51.3.577

Partial Characterization of Cx Cellulase and Cellobiase from Ripening Tomato Fruits 1

D M Pharr a,2, D B Dickinson a
PMCID: PMC366307  PMID: 16658371

Abstract

Cellulolytic enzymes were studied in extracts from the locular contents of ripening fruits of Lycopersicon esculentum var. KC-146. When acting on carboxymethyl cellulose, the enzyme preparations were capable of decreasing the viscosity of the reaction mixture and generating reducing groups, oligosaccharides, and glucose. Cellobiose cellotriose, cellotetrose, and cellopentose also served as substrates for glucose production.

These properties were due to the presence in the extracts of two cellulolytic enzymes—Cx cellulase (E.C. 3.2.1.4) and cellobiase— (E.C. 3.2.1.21). No evidence was found for the presence of exocellulase or C1, an enzyme which initiates enzymatic attack on insoluble cellulose.

Cellobiose was found to be weakly inhibitory to Cx cellulase. d-Glucono-1,5-lactone competitively inhibited cellobiase. The Km for cellobiose was 0.34 mm, and the Ki for gluconolactone was 23 μM.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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