Table 3. Thermodynamic comparison of wild type, CP49/48 and CP50/49 at 310 K.

	Wild type	CP49/48	CP50/49
K a ratio (mutant:wt)	—	—	417 ± 14
ΔΔG° (kcal mol−1)	—	—	+3.7 ± 0.1
ΔΔH° (kcal mol−1)	—	+7.9 ± 0.1	+7.6 ± 0.1
TΔΔS° (kcal mol−1)	—	—	+3.8 ± 0.1
ΔC p (cal mol−1 K−1)	−345 ± 12	−166 ± 3	−145 ± 3
T melting (K)	352	—	360
k off (s−1)	2.9 × 10−5	0.33	0.024
ΔG ‡ (kcal mol−1)	24.6	18.8 ± 1.3	20.5 ± 0.9
ΔH ‡ (kcal mol−1)	30.4	39.3 ± 0.6	29.5 ± 0.4
TΔS ‡ (kcal mol−1)	5.8	20.5 ± 0.7	9.0 ± 0.5