Structures of the superimposed chromophores of mKate (Pletnev et al., 2008 ▶) and phiYFPv with the isomer-stabilizing residues shown. mKate: fluorescent cis form at pH 7.0, pink; nonfluorescent trans form at pH 2.0, dark grey. phiYFPv: fluorescent cis form at pH 8.0, yellow; hypothetical modelled nonfluorescent trans form, light grey. This figure was produced using PyMOL (DeLano, 2002 ▶).