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Coculturing keto-MA-deficient strains does not restore pellicle growth. H37Rv, ΔmmaA4, and H37Rv::mmaA3 strains, individually and in combinations—ΔmmaA4 and H37RV coculture, H37RV::mmaA3 and H37Rv coculture, H37RV::mmaA3 and ΔmmaA4 coculture—were inoculated into Sauton’s medium without Tween 80 and incubated for 3 weeks under pellicle-promoting conditions.
