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. 2013 May 7;4(3):e00222-13. doi: 10.1128/mBio.00222-13

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Copyright © 2013 Sambandan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 5 — Analysis of mycolic acids under pellicle-promoting conditions. Major apolar lipids from cells grown as pellicles were derivatized as methyl esters and resolved on TLC using petroleum ether-acetone (95:5 [vol/vol]). (A) Methoxy-MA (M) is enriched in the wild-type pellicle (lane 1). The mmaA4 extract lacks both keto-MA (K) and methoxy-MA (lane 2). This defect is relieved in the complemented strain (lane 3). (B) The H37Rv::mmaA3 extract (lane 2) is deficient in keto-mycolates relative to the wild-type H37Rv (lane 1) but retains wild-type levels of methoxy-MA. O, origin; FAME, fatty acid methyl esters; MAME, mycolic acid methyl esters. This shows that methoxy mycolate, despite being a major constituent of mycobacterial pellicle, is dispensable for growth as a pellicle, and keto-mycolate, despite being a minor constituent, is essential.