TABLE 1 .
Quantitative analysis of subcellular cluster formation in strains expressing different YFP-tagged WspR variants
| Mutation | Location | % cells with clusters (n) for genotypea |
|||||
|---|---|---|---|---|---|---|---|
| Broth grown |
Agar grown |
||||||
| ΔwspA | WT | ΔwspF | ΔwspA | WT | ΔwspF | ||
| None | 1 (715) | 1 (295) | 34 (493) | 0 (339) | 43 (411) | 77 (494) | |
| D70A | P site | 0 (250) | 0 (178) | 0 (138) | 0 (256) | 0 (190) | 0 (139) |
| V72D | D + 2b | 2 (228) | 2 (572) | 0 (256) | 0 (99) | 0 (599) | 1 (310) |
| L167D | Linker stalk | 0 (158) | 0 (506) | 23 (381) | 0 (288) | 4 (684) | 72 (632) |
| L170D | Linker stalk | 0 (604) | 29 (632) | 82 (222) | 1 (266) | 75 (468) | 72 (186) |
| R198A | I site | 0 (173) | 9 (265) | 36 (140) | 0 (128) | 38 (366) | 48 (261) |
| E253A | Cyclase active site | 0 (266) | 12 (145) | 75 (232) | 0 (194) | 68 (276) | 75 (130) |
a
Percentages represent the numbers of cells with at least one well-defined fluorescent spot divided by the total number of visualized cells (n), shown in parentheses. A well-defined fluorescent spot is defined by dividing the maximum pixel intensity by the average pixel intensity in the cell. All cells with a resultant number above an empirically determined threshold are considered cells with at least one cluster. WT, wild type.
b
Two residues downstream of conserved aspartate at the P-site.