TABLE 2 .
Strains used in this study
| Strain | Source or reference |
|---|---|
| Pseudomonas aeruginosa | |
| PAO1 | 35 |
| PAO1 wspR-yfp | 12 |
| PAO1 ΔwspR | 7 |
| PAO1 ΔwspFR | 7 |
| PAO1 ΔwspAR | 12 |
| PAO1 ΔwspR attB::miniCTX-wspR-yfpa | This study |
| PAO1 ΔwspR attB::miniCTX-wspRD70A-yfp | This study |
| PAO1 ΔwspR attB::miniCTX-wspRV72D-yfp | This study |
| PAO1 ΔwspR attB::miniCTX-wspRL167D-yfp | This study |
| PAO1 ΔwspR attB::miniCTX-wspRL170D-yfp | This study |
| PAO1 ΔwspR attB::miniCTX-wspRR198A-yfp | This study |
| PAO1 ΔwspR attB::miniCTX-wspRE253A-yfp | This study |
| PAO1 ΔwspF wspR-yfp | 12 |
| PAO1 ΔwspFR attB::miniCTX-wspR-yfp | This study |
| PAO1 ΔwspFR attB::miniCTX-wspRD70A-yfp | This study |
| PAO1 ΔwspFR attB::miniCTX-wspRV72D-yfp | This study |
| PAO1 ΔwspFR attB::miniCTX-wspRL167D-yfp | This study |
| PAO1 ΔwspFR attB::miniCTX-wspRL170D-yfp | This study |
| PAO1 ΔwspFR attB::miniCTX-wspRR198A-yfp | This study |
| PAO1 ΔwspFR attB::miniCTX-wspRE253A-yfp | This study |
| PAO1 ΔwspA wspR-yfp | 12 |
| PAO1 ΔwspAR attB::miniCTX-wspR-yfp | This study |
| PAO1 ΔwspAR attB::miniCTX-wspRD70A-yfp | This study |
| PAO1 ΔwspAR attB::miniCTX-wspRV72D-yfp | This study |
| PAO1 ΔwspAR attB::miniCTX-wspRL167D-yfp | This study |
| PAO1 ΔwspAR attB::miniCTX-wspRL170D-yfp | This study |
| PAO1 ΔwspAR attB::miniCTX-wspRR198A-yfp | This study |
| PAO1 ΔwspAR attB::miniCTX-wspRE253A-yfp | This study |
| Escherichia coli | |
| DH5α | Gibco-BRL |
| S17-1 | American Type Culture Collection |
| Rosetta 2 | Novagen |
a
The miniCTX backbone of pTG142 was integrated in the chromosome alongside wspR. See Materials and Methods and reference 34.