TABLE 3 .
Sequence identities of synthetic peptides with CT-B and their ELISA reactivities with rabbit CT antiserum
| Peptide | Peptide sequencea | % identity with CT-B |
ELISA reactivity ratiob |
|---|---|---|---|
| P1 | 1-MKLVKLSLVAALAAGAFSAANATPLEEAIK-30 | 26.7 | 1.37 |
| P2 | 21-NATPLEEAIKDVDVSGVLRYRYDTGNFDKN-50 | 26.7 | 1.14 |
| P3 | 41-RYDTGNFDKNFVNNSNLNNSKQDHKYRAQV-70 | 10.0 | 1.23 |
| P4 | 61-KQDHKYRAQVNFSAAIADNFKAFVQFDYNA-90 | 23.3 | 1.06 |
| P5 | 81-KAFVQFDYNAADGGYGANGIKNDQKGLFVR-110 | 16.7 | 1.21 |
| P6 | 101-KNDQKGLFVRQLYLTYTNEDVATSVIAGKQ-130 | 26.7 | 1.15 |
| P7 | 121-VATSVIAGKQQLNLIWTDNAIDGLVGTGVK-150 | 10.0 | 1.26 |
| P8 | 141-IDGLVGTGVKVVNNSIDGLTLAAFAVDSFM-170 | 26.7 | 1.30 |
| P9 | 161-LAAFAVDSFMAAEQGADLLEHSNISTTSNQ-190 | 13.3 | 1.21 |
| P10 | 181-HSNISTTSNQAPFKVDSVGNLYGAAAVGSY-210 | 30.0 | 0.96 |
| P11 | 201-LYGAAAVGSYDLAGGQFNPQLWLAYWDQVA-230 | 33.3 | 1.06 |
| P12 | 221-LWLAYWDQVAFFYAVDAAYSTTIFDGINWT-250 | 23.3 | 1.10 |
| P13 | 241-TTIFDGINWTLEGAYLGNSLDSELDDKTHA-270 | 20.0 | 1.15 |
| P14 | 261-DSELDDKTHANGNLFALKGSIEVNGWDASL-290 | 33.3 | 1.06 |
| P15 | 281-IEVNGWDASLGGLYYGDKEKASTVVIEDQG-310 | 20.0 | 1.23 |
| P16 | 301-ASTVVIEDQGNLGSLLAGEEIFYTTGSRLN-330 | 23.3 | 2.15 |
| P17 | 321-IFYTTGSRLNGDTGRNIFGYVTGGYTFNET-350 | 30.0 | 0.96 |
| P18 | 341-VTGGYTFNETVRVGADFVYGGTKTEAANHL-370 | 20.0 | 0.99 |
| P19 | 361-GTKTEAANHLGGGKKLEAVARVDYKYSPKL-390 | 20.0 | 1.09 |
| P20 | 381-RVDYKYSPKLNFSAFYSYVNLDQGVNTNES-410 | 16.7 | 1.13 |
| P21 | 401-LDQGVNTNESADHSTVRLQALYKF-424 | 13.3 | 1.06 |
a
The amino acids are denoted by single-letter designation. The positions of amino acids at the beginning and end of peptides are numbered; amino acids that overlap adjacent peptides are underlined. Numbering is based on the sequence of the major outer membrane protein (MOMP) of C. jejuni NCTC 11168.
b
The OD reading at a wavelength of 405 nm for postimmunization CT antiserum was divided by that for preimmune serum.