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RT-qPCR validation of candidates in dilution series of RBCs in plasma. (A) Previously identified hemolysis affected microRNAs (B) additional hemolysis affected microRNAs (C) RBC- and hemolyzed plasma specific microRNAs (D) unaffected microRNAs. Data are presented as raw Cq value ± SD obtained from measurements in RNA isolated from two independent dilution series.
